Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.Density 1.077 g/ml.Best alternative to Ficoll^®.

ROTI^®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll^® this reagent may be replaced by ROTI^®Sep 1077 human without any adjustment.