NSFL1C Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8346-50, BS8346-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UNZ2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB 1:500 - 1:2000 IF 1:50 - 1:200

Background: p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NSFL1C polyclonal antibody detects endogenous levels of NSFL1C protein.

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: WesternBlot (WB) analysis of NSFL1C polyclonal antibody, Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody.

Alternative Name: NSFL1 cofactor p47; UBX domain-containing protein 2C; p97 cofactor p47; NSFL1C; UBXN2C

Immunogen: Recombinant full length Human NSFL1C.

Conjugate: Unconjugated

Modification: Unmodified