TRP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91379-50, BS91379-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17643(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:500-1:2,000
ICC:1:50-1:100
IHC:1:50-1:200
IP:1:10-1:50

Background: Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TRP1 polyclonal antibody detects endogenous levels of TRP1 protein.

Bioworld Molecular Weight: 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution., ICC staining TRP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: DHICA oxidase, TRP, TRP-1, TRP1, TYRP1, CAS2, TYRP, TYRRP, 5, 5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, b-PROTEIN, CAS2, Catalase B, CATB, DHICA oxidase, Glycoprotein 75, GP75, Melanoma antigen gp75, OCA3, TRP, TRP-1, TRP1, Tyrosinase related protein 1, Tyrosinase-related protein 1, TYRP, TYRP1, TYRP1, TYRRP,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified