RhoA/B/C Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ17008-50, BZ17008-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P61586/P62745/P08134

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

Background: RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RhoA/B/C in Jurkat lysates using RhoA/B/C antibody., Immunocytochemistry analysis of Rho A/B/C in Hela using Rho A/B/C antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using RhoA/B/C antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC

Immunogen: Recombinant protein of human Rho C

Conjugate: Unconjugated

Modification: Unmodified