YY1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91461-50, BS91461-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25490(Human) Q00899(Mouse) EntrezGene:24919(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain μE1 site as well as the P5 promoter of the adeno-associated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: YY1 polyclonal antibody detects endogenous levels of YY1 protein.

Bioworld Molecular Weight: 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of YY1 on different lysates using anti-YY1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: HL-60, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YY1 antibody. Counter stained with hematoxylin.

Alternative Name: YY-1, CF1, Delta, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, NF-E1, NFE1, OTTHUMP00000197459, Transcriptional repressor protein YY1, TYY1, UCR motif DNA binding protein, UCRBP, Yin and yang 1, Yin and Yang 1 protein, Yin Yang 1, Ying Yang 1, YY 1, YY 1 transcription factor, YY-1, YY1, YY1 transcription factor,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified