GPR20 Polyclonal Antibody

Catalogue Numbers: BS5746-50, BS5746-100

Sizes: 50µl, 100µl

Alternative Name: G-protein coupled receptor 20

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99678

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. G protein-coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR20 is a 358 amino acid membrane protein that constitutively activates G(i) proteins without ligand stimulation. Also, GPR20 may be involved in the control of intracellular cAMP levels and mitogenic signaling. Interestingly, GPR20 is expressed in liver and certain regions of the brain, including putamen, caudate and thalamus, but is not expressed in hypothalamus, pons and frontal cortex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR20 polyclonal antibody detects endogenous levels of GPR20 protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GPR20 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 313-358 of Human GPR20.

Conjugate: Unconjugated

Modification: Unmodified