AhR (K32) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1791-50, BS1791-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35869

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
IP: 1:50~1:200

Background: The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: AhR (K32) polyclonal antibody detects endogenous levels of AhR protein.

Bioworld Molecular Weight: ~ 94 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of AhR (K32) polyclonal antibody at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of AhR (K32) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunoprecipitation of SGC7901 cell lysate using AhR (K32) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3). Lane 1 is 30% input. The western blot was probed using AhR (K32) #BS1791. “↑”（supernatant）;“↓(deposition）

Alternative Name: Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human AhR.

Conjugate: Unconjugated

Modification: Unmodified