Recombinant Human/Cynomolyus CD28/TP44 (C-Fc-Avi) Biotinylated

Sizes: 20μg, 100μg

Catalogue Numbers: PKSH033948-20, PKSH033948-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD28; TP44

Target Symptom: CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44

Research Area: immunology; Stem cells

Conjugation: Biotinylated

Target Species: Human/Cynomolyus

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc-Avi

UNIProt ID: P10747

Accession: P10747

Background: T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Activity: Immobilized Anti-CD28 mAb at 2μg/ml (100 μl/well) can bind Human/Cynomolgus CD28-Fc-Avi. The ED50 of Human/Cynomolgus CD28-Fc-Avi is 0.84 ng/ml.

Sequence: Asn19-Pro152

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mTris-HCl, 150mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.1 kDa

Observed MW: 60-90 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only