Glucosidase IIβ (K117) Polyclonal Antibody

Catalogue Numbers: BS2646-50, BS2646-100

Sizes: 50µl, 100µl

Alternative Name: Glucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH; PRKCSH; G19P1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14314

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ). The α and β subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIα is the catalyitc core of the enzyme and can function independently of the β subunit. The sequence of Glucosidase IIβ encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIβ is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIβ was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIβ retains the complete complex in the ER.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Glucosidase IIβ (K117) polyclonal antibody detects endogenous levels of Glucosidase IIβ protein.

Bioworld Molecular Weight: ~ 59, 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Glucosidase IIβ (K117) pAb at 1:500 dilution
Lane 1: The Testis tissue lysate of Mouse(40ug)
Lane 2: The Testis tissue lysate of Rat(40ug)
Lane 3: SGC7901 whole cell lysate(40ug)
Lane 4: HCT116 whole cell lysate(40ug)
Lane 5: A549 whole cell lysate(40ug)
Lane 6: MCF-7 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase IIβ.

Conjugate: Unconjugated

Modification: Unmodified