MAL Polyclonal Antibody

Catalogue Numbers: BS60179-50, BS60179-100

Sizes: 50µl, 100µl

Alternative Name: Myelin and lymphocyte protein; T-lymphocyte maturation-associated protein; MAL

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21145

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The MAL-SRF interaction displays the predicted properties of a Rho-regulated SRF cofactor. MAL is predominantly cytoplasmic in serum-starved cells, but accumulates in the nucleus following serum stimulation. Activation of the Rho-actin signaling pathway is necessary and sufficient to promote MAL nuclear accumulation. MAL N-terminal sequences, including two RPEL motifs, are required for the response to signaling, while other regions mediate its nuclear export (or cytoplasmic retention) and nuclear import. MAL associates with unpolymerized actin through its RPEL motifs. Constitutively cytoplasmic MAL derivatives interfere with MAL redistribution and Rho-actin signaling to SRF.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MAL pAb detects endogenous levels of MAL protein.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MAL pAb at 1:500 dilution
Lane 1: Myla2059 whole cell lysate(40ug)
Lane 2: HuT78 whole cell lysate(40ug)
Lane 3: The Spleen tissue lysate of Rat(40ug)
Lane 4: The Spleen tissue lysate of Mouse(40ug)
Lane 5: AML-12 whole cell lysate(40ug)
Lane 6: CT26 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding Human MAL.

Conjugate: Unconjugated

Modification: Unmodified