Recombinant Human KEAP1/INRF2 Protein

Size: 100μg

Catalogue Numbers: PKSH030943-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: KEAP1; INRF2

Target Synonym: INRF2; KEAP-1; KLHL19

UNIProt ID: Q14145

Research Areas: epigenetics and nuclear signal

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: None

Accession: Q14145

Background: Kelch-like ECH-associated protein 1; also known as cytosolic inhibitor of Nrf2; Kelch-like protein 19; KEAP1 and INRF2; is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain; one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed; with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor; which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions; KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome; thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.

Sequence: Gln2-Cys624

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 69.7 kDa

Observed MW: 64 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only