Human DBP (Vitamin D-binding protein) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-H6167-24, E-EL-H6167-48, E-EL-H6167-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: DBP

UNIProt ID: P02774

Target Synonyms: Vitamin D-binding protein; gc-globulin; GC; DBP; DBP/GC; GRD3; VDBG; VDBP;

Research Areas: Cancer, Metabolism, Signaling transduction

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human DBP concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.09 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DBP. You can calculate the concentration of Human DBP in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag