CYFIP1 Polyclonal Antibody

Catalogue Numbers: BS60773-50, BS60773-100

Sizes: 50µl, 100µl

Alternative Name: ytoplasmic FMR1-interacting protein 1; Specifically Rac1-associated protein 1; Sra-1; p140sra-1; CYFIP1; KIAA0068

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7L576

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Fragile X syndrome is the most frequent form of inherited mental retardation and is a result of transcriptional silencing of the FMR1 gene on the X chromosome. The FMR1 protein (also designated FMRP) is an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. FMR1 can also interact with two fragile X syndrome related factors, FXR1 (also designated FXR1P) and FXR2 (also designated FXR2P). These proteins form heterodimers through their N-terminal coiled-coiled domains. CYFIP1 and CYFIP2 (also known as cytoplasmic FMRP interacting proteins 1 and 2, respectively, and as Sra-1 in mouse) both interact with FMR1 but CYFIP2 also reacts with FXR1 and FXR2. CYFIP1 and CYFIP2 bind GTP-bound Rac1 to release FMRP in its active state, which is thought to regulate mRNA translation of neural cytoskeletal proteins. A loss of CYFIP1 and CYFIP2 leads to mutant neurons with defective axonal growth and motor function.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYFIP1 polyclonal antibody detects endogenous levels of CYFIP1 protein.

Bioworld Molecular Weight: ~ 145 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYFIP1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CYFIP1

Conjugate: Unconjugated

Modification: Unmodified