Recombinant Human CD3E & CD3G Heterodimer Protein (Active)

Size: 50μg

Catalogue Number: PKSH030476-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD3E & CD3G Heterodimer

Target Symptom: CD3E; CD3G Heterodimer; IMD18; T3E; TCRE

Research Area: immunology; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-hFc-Flag & mFc Tag

Accession: NP_000724.1&NP_000064.1

Background: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Activity: Measured by its ability to bind OKT3-mIgG2a in a functional ELISA.

Sequence: Met 1-Asp126&Met1-Ser116

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 77.2 (40.1+37.1) kDa

Observed MW: 42-47 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only