MYO10 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71231-50, BS71231-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9HD67

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 270kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MYO10 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s., Immunofluorescence analysis of U2OS cells using MYO10 antibody at dilution of 1:350. Blue: DAPI for nuclear staining., Immunofluorescence analysis of A-549 cells using MYO10 antibody at dilution of 1:350. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using MYO10 antibody at dilution of 1:350. Blue: DAPI for nuclear staining.

Alternative Name: MYO10; myosin X

Immunogen: Recombinant fusion protein of human MYO10(NP_036466.2).

Conjugate: Unconjugated

Modification: Unmodified