GK Polyclonal Antibody

Catalogue Numbers: BS5733-50, BS5733-100

Sizes: 50µl, 100µl

Alternative Name: Glycerolkinase; Glycerol kinase; Glycerokinase; ATP:glycerol 3-phosphotransferase

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P32189

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: GK is a 559 amino acid mitochondrial peripheral membrane protein that belongs to the FGGY kinase family and is a key enzyme involved in the regulation of glycerol uptake and metabolism. GK shows high expression in kidney, testis and liver and exists as three isoforms, which are produced as a result of alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GK polyclonal antibody detects endogenous levels of GK protein.

Bioworld Molecular Weight: ~ 61 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GK polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 500-545 of Human GK.

Conjugate: Unconjugated

Modification: Unmodified