DCAMKL1/DCLK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79230-50, BS79230-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15075

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:20 - 1:50

Background: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 82KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using DCAMKL1/DCLK1 antibody at 1:850 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of Rat brain using DCAMKL1/DCLK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of Mouse brain using DCAMKL1/DCLK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of rat brain cells using DCAMKL1/DCLK1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: DCLK1; CL1; CLICK1; DCAMKL1; DCDC3A; DCLK

Immunogen: Recombinant fusion protein of human DCAMKL1/DCLK1(NP_001317000.1).

Conjugate: Unconjugated

Modification: Unmodified