Recombinant Human BPGM Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032119-10, PKSH032119-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: BPGM

Target Synonym: Bisphosphoglycerate Mutase; BPGM; 2,3-Bisphosphoglycerate Mutase Erythrocyte; 2,3-Bisphosphoglycerate Synthase; 2,3-Diphosphoglycerate Mutase; DPGM; BPG-Dependent PGAM; BPGM

UNIProt ID: P07738

Research Areas: Signal Transduction; Cardiovascular; Cancer; metabolism;

Target Species: Human

Expression Host: E.coli

Fusion Tag: C-6His

Accession: P07738

Background: Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily. BPGM is a multifunctional enzyme. BPGM catalyzes 2, 3-DPG synthesis via its synthetase activity, and 2, 3-DPG degradation via its phosphatase activity. It also has phosphoglycerate phosphomutase activity. BPGM plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2, 3-bisphosphoglycerate (2, 3-BPG). Deficiency of BPGM increases the affinity of cells for oxygen and result in hemolytic anemia.

Sequence: Ser2-Lys259

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 1mM DTT, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 31.0 kDa

Observed MW: 30 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only