Mouse SCCA2 (Squamous Cell Carcinoma Antigen 2) CLIA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-CL-M0618-24, E-CL-M0618-48, E-CL-M0618-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: SCCA2

UNIProt ID: Q9Z2G2 Research Areas: Cancer, Cell Biology, Immunology

Target Species: Mouse

Application: CLIA

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse SCCA2 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 7.5 pg/mL

Detection Range: 12.5-800 pg/mL

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse SCCA2. You can calculate the concentration of Mouse SCCA2 in the samples by comparing the RLU value of the samples to the standard curve.

Cross Reactivity: This kit recognizes Mouse SCCA2 in samples. No significant cross-reactivity or interference between Mouse SCCA2 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 6 months

Shipping: ice bag