Pumilio 1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91138-50, BS91138-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14671(Human) Q80U78(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IF, IHC FC IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Pumilio 1 polyclonal antibody detects endogenous levels of Pumilio 1 protein.

Bioworld Molecular Weight: 126 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1,000 dilution., ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1, HsPUM, KIAA0099, PUM 1, PUM L1, Pum1, PUM1, PUMH 1, PUMH, PUMH1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, Pumilio-1, Pumilio1, PUML 1, PUML1,

Immunogen: Recombinant protein within human Pumilio 1 aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified