CA IX (D82) Polyclonal Antibody

Catalogue Numbers: BS2975-50, BS2975-100

Sizes: 50µl, 100µl

Alternative Name: Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250 pMW1; CA9; G250; MN

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16790

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: CAs are involved in a variety of biological processes, including respiration, calcification,acid-base balance, bone resorption and the formation of aqueous humor,cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA9,CA12 and CA14 genes, which map to chromosomes 9p13.3, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CA IX (D82) polyclonal antibody detects endogenous levels of CA IX protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CA IX (D82) pAb at 1:500 dilution
Lane 1: C6 whole cell lysate(40ug)
Lane 2: CT26 whole cell lysate(40ug)
Lane 3: PC3 whole cell lysate(40ug)
Lane 4: Hela whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 50-104 of Human CA IX.

Conjugate: Unconjugated

Modification: Unmodified