Nesprin 1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90929-50, BS90929-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NF91(Human) Q6ZWR6(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:2,000
ICC:1:200-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Background: Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Nesprin 1 polyclonal antibody detects endogenous levels of Nesprin 1 protein.

Bioworld Molecular Weight: 112 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: Mouse spleen tissue, ICC staining Nesprin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: KASH1, Myne-1, Syne-1, SYNE1, C6orf98, KIAA0796, KIAA1262, KIAA1756, MYNE1, 8B, ARCA1, C6orf98, CPG2, CPG2 full length, dJ45H2.2, EDMD4, Enaptin, Myne-1, MYNE1, Myocyte nuclear envelope protein 1, Nesp1, Nesprin-1, Nuclear envelope spectrin repeat protein 1, SCAR8, Spectrin repeat containing nuclear envelope 1, Synaptic nuclear envelope protein 1, Synaptic nuclei expressed gene 1, Syne-1, SYNE1, SYNE1, SYNE1B,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified