Kir5.1 (phospho-S416) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67195-50, BS67195-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9NPI9

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC, IF/IC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (PubMed:24561201).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 156% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Kir5 protein.1 with a phosphorylation site at S416 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Kir5.1 (pS416) expression in HEK293T (A), HuT78 (B), mouse spleen (C), rat spleen (D) whole cell lysates., Immunohistochemical analysis of Kir5.1 (pS416) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of Kir5.1 (pS416) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: KCNJ16; Inward rectifier potassium channel 16; Inward rectifier K(+) channel Kir5.1; Potassium channel inwardly rectifying subfamily J member 16

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Kir5.1 with a phosphorylation site at S416. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Phosphorylation