Recombinant Human IL1R1/CD121a Protein (Fc Tag)(Active) Size: 100μg Catalogue Number: PKSH031849-100 Citations, Manuals and MSDS Available upon request. Abbreviation: IL1R1; CD121a Target Symptom: CD121A; D2S1473; IL-1 RI; IL-1R-alpha; IL-1R1; IL1R; IL1RA; P80; Interleukin-1 receptor type 1; IL-1R-1; IL-1RT-1; IL-1RT1; CD121 antigen-like family member A; Interleukin-1 receptor alpha; IL-1R-alpha Research Area: Cardiovascular; immunology; Stem cells Target Species: Human Expression Host: HEK293 Cells Application: ELISA Fusion Tag: C-hFc Accession: NP_000868.1 Background: Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess. Activity: 1. Immobilized human IL1-beta at 20 μg/ml (100 μl/well) can bind human IL1R1 Fc chimera with a linear ranger of 6. 4-160 ng/ml. 2. Immobilized human IL1B-his at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc. The EC50 of human IL1R1-Fc is 0.198 μg/mL. Sequence: Met 1-Thr 332 Purity: > 90 % as determined by reducing SDS-PAGE. Formulation: Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. Reconstitution: Please refer to the printed manual for detailed information. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Calculated MW: 63 kDa Observed MW: 80-90 kDa Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shipping: This product is provided as lyophilized powder which is shipped with ice packs. Research Use Only