FOXO4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90533-50, BS90533-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P98177(Human) Q9WVH3(Mouse) Unigene:19646(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP, , FC

All Applications: WB:1:1,000
FC:1:50-1:100
ICC:1:100-1:500

Background: FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serine-proline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x;11) translocation of the AFX1 gene, which involves the fusion of a serine-proline-rich sequence of AFX1 to the carboxy terminus of a truncated MLL, results in acute lymphocytic leukemia.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: FOXO4 polyclonal antibody detects endogenous levels of FOXO4 protein.

Bioworld Molecular Weight: 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining FOXO4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining FOXO4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: FOXO4, AFX, AFX1, Afxh, ALL1-fused gene from X chromosome, Fork head domain transcription factor AFX1, Forkhead box O4, Forkhead box protein O4, FOXO 4, Foxo4, FOXO4, MGC117660, MGC120490, Mixed lineage leukemia, translocated to, 7, MLLT7, Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7, Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7, RGD1561201,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified