ATP1A2 Polyclonal Antibody

Catalogue Numbers: BS3401-50, BS3401-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P50993

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP1A2 polyclonal antibody detects endogenous levels of ATP1A2 protein.

Extra Notes: Western blot (WB) analysis of ATP1A2 polyclonal antibody at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:H1792 whole cell lysate(40ug)
Lane3:Panc1 whole cell lysate(40ug)
Lane4:Aml-12 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 95kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Sodium/potassium-transporting ATPase subunit alpha-2;Na(+)/K(+) ATPase alpha-2 subunit;Sodium pump subunit alpha-2;ATP1A2;KIAA0778;

Immunogen: Synthetic peptide, corresponding to Human ATP1A2 .

Conjugate: Unconjugated

Modification: Unmodified