Adducin γ (P452) Polyclonal Antibody

Catalogue Numbers: BS2209-50, BS2209-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UEY8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain andhematopoietic tissues, such as bone marrow in humans, and spleen in mice.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Adducin γ (P452) polyclonal antibody detects endogenous levels of Adducin γ protein.

Extra Notes: Western blot (WB) analysis of Adducin γ (P452) polyclonal antibody at 1:500 dilution
Lane1:BV2 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:LO2 whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:HepG2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 82 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: ADD3; ADDG; ADDL; Adducin 3 (gamma); Adducin 3; Adducin-like protein 70; Gamma-adducin; Adducinγ

Immunogen: Synthetic peptide, corresponding to amino acids 420-471 of Human Adducin γ.

Conjugate: Unconjugated

Modification: Unmodified