Mib1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90857-50, BS90857-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q86YT6(Human)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC

All Applications: ICC:1:50-1:200
IHC:1:50-1:200
WB:1:500

Background: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Mib1 polyclonal antibody detects endogenous levels of Mib1 protein.

Bioworld Molecular Weight: 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Mib1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Mib1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: DIP-1, MIB1, DIP1, KIAA1323, ZZANK2, DAPK-interacting protein 1, Dip 1, DIP-1, Dip1, E3 ubiquitin protein ligase MIB 1, E3 ubiquitin protein ligase MIB1, E3 ubiquitin-protein ligase mib1, KIAA1323, LVNC7, MIB, mib1, MIB1, Mind bomb homolog 1, Mindbomb E3 ubiquitin protein ligase 1, Ubiquitin ligase mind bomb, Zinc finger ZZ type with ankyrin repeat domain protein 2, ZZANK2, ZZZ6,

Immunogen: Recombinant protein within N-terminal human Mib1.

Conjugate: Unconjugated

Modification: Unmodified