IAP Polyclonal Antibody

Catalogue Numbers: BS60372-50, BS60372-100

Sizes: 50µl, 100µl

Alternative Name: Intestinal-type alkaline phosphatase; Intestinal alkaline phosphatase; ALPI

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09923

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The intestinal alkaline phosphatase gene (ALPI) encodes a digestive brush-border enzyme, IAP (also designated ALP), which is highly upregulated during small intestinal epithelial cell differentiation. IAP, an enterocyte differentiation marker that functions to limit fat absorption, has been implicated in trans-cellular transport of chylomicrons and in chylomicron formation. At high pH, IAP removes phosphate groups from proteins and from the 5' end of DNA and RNA. Most mammals have four different IAP isozymes: placental, placental- like, intestinal and non tissue-specific. Non tissue-specific isozymes are found in liver, kidney and bone. Tissues with particularly high concentrations of IAP include the liver, bile ducts, placenta and bone.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IAP polyclonal antibody detects endogenous levels of IAP protein.

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IAP polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human IAP.

Conjugate: Unconjugated

Modification: Unmodified