GSDMD-CT Rabbit Monoclonal Antibody

Catalogue Numbers: MB66353-50, MB66353-100

Sizes: 50µl, 100µl

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: P57764

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis. Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18. Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GSDMD-CT protein.

Extra Notes: Western blot analysis of GSDMD-CT expression in Jurkat (A) whole cell lysates.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DFNA5L; GSDMDC1; Gasdermin-D; Gasdermin domain-containing protein 1

Immunogen: Recombinant protein of human GSDMD

Conjugate: Unconjugated

Modification: Unmodified