14-3-3 epsilon Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90002-50, BS90002-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62258(Human) P62259(Mouse) P62260(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100

Background: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: 14-3-3 epsilon polyclonal antibody detects endogenous levels of 14-3-3 epsilon protein.

Bioworld Molecular Weight: 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY-5Y
Lane 2: 293T, ICC staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: 14-3-3E, YWHAE, 14 3 3 E, 14 3 3 epsilon, 14 3 3E, 14-3-3 protein epsilon, 14-3-3E, 1433E, Epididymis luminal protein 2, FLJ45465, FLJ53559, HEL2, KCIP 1, KCIP1, MDCR, MDS, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, YWHAE,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified