Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein (Active)

Size: 50μg

Catalogue Number: PKSH030312-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: AMPK Heterotrimer

Target Symptom: AMPK; AMPKa1

Research Area: Signal Transduction; Cardiovascular; Cancer; metabolism;

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion Tag: N-His & GST Tag

UNIProt ID: P54619&Q9Y478&P54646

Accession: P54619&Q9Y478&P54646

Activity: The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Sequence: Met 1-Pro 331&Met 1-Ile 272&Met 1-Arg 552

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% glycerol, 1mM GSH, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 158 (38+30+90) kDa

Observed MW: 35; 37 & 95 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only