UM171, CAS No. 1448724-09-1, is a potent small molecule that stimulates the ex vivo expansion of human hematopoietic stem cells/HSC capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. It attenuates cell differentiation and promotes ex vivo expansion of long-term human HSC (LT-HSC) at 35 nM in 11 days fed-batch cultures. UM171’s mechanism is independent of suppression of the aryl hydrocarbon receptor (AHR) like SR1. The properties of UM171 make it a potential candidate for hematopoietic stem cell transplantation and gene therapy.

Product information

CAS Number: 1448724-09-1

Molecular Weight: 526.46

Formula: C25H29Cl2N9

Synonym:

UM-171

UM 171

Related CAS Number:

1448724-09-1 (free base)

Chemical Name: (1r,4r)-N1-(2-benzyl-7-(2-methyl-2H-tetrazol-5-yl)-9H-pyrimido[4,5-b]indol-4-yl)cyclohexane-1,4-diamine hydrochloride

Smiles: Cl.Cl.CN1N=C(N=N1)C1=CC=C2C(=C1)NC1=NC(CC3C=CC=CC=3)=NC(N[C@@H]3CC[C@@H](N)CC3)=C21

InChiKey: HMZHPVMDZWVCJD-PMXFUEHMSA-N

InChi: InChI=1S/C25H27N9.2ClH/c1-34-32-23(31-33-34)16-7-12-19-20(14-16)28-25-22(19)24(27-18-10-8-17(26)9-11-18)29-21(30-25)13-15-5-3-2-4-6-15;;/h2-7,12,14,17-18H,8-11,13,26H2,1H3,(H2,27,28,29,30);2*1H/t17-,18-;;

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: Powder: 4^oC 1 year. DMSO: 4^oC 3 month; -20^oC 1 year.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

For transplantation experiments, the fed-batch culture system was used. 1x105 CD34+ cord blood (CB) cells/ml were injected into 12 ml or 25 ml bags connected to a syringe loaded pumping system and maintained on an orbital shaker at 37°C and 5% CO2 in air. The pump was set to continuously deliver HSC expansion media supplemented with UM171 (35 nM) at a flow rate of 0.7 μl/min.

In Vivo:

NSG mice were injected with CD34+ CB cells that had been originally cultured in UM171.

References:

1. Fares I, et al. Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. (2014) Science. 345(6203):1509-12.

Products are for research use only. Not for human use.

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