ERK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90473-50, BS90473-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28482

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000-1:2,000
ICC:1:200
IHC:1:200
FC:1:100-1:200

Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: ERK2 polyclonal antibody detects endogenous levels of ERK2 protein.

Bioworld Molecular Weight: 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ERK2 on different cell lysates using anti- ERK2 antibody at 1/2000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: A431
Lane 3: PC12
Lane 4: NIH/3T3
Lane 5: Hela
Lane 6: HT-29
Lane 7: MCF-7, ICC staining of ERK2 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2, ERK 1, ERK 2, ERK-2, ERK1, erk1/2, ERK2, ERT1, ERT2, Extracellular signal regulated kinase 1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Mapk1, MAPK2, MAPK3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01, p38, p40, p41, p42-MAPK, PRKM 2,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified