DDIT4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90402-50, BS90402-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NX09(Human) Q9D3F7(Mouse) Q8VHZ9(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100

Background: REDD-1, also designated DNA-damage-inducible transcript 4, dig2 or RTP801, is thought to function in the regulation of reactive oxygen species (ROS). REDD-1 expression has also been linked to apoptosis, Ab toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level. REDD-1 negatively regulates the mammalian target of Rapamycin (mTOR), a serine/threonine kinase often referred to as FRAP. It is crucial in the coupling of extra- and intracellular cues to FRAP regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: DDIT4 polyclonal antibody detects endogenous levels of DDIT4 protein.

Bioworld Molecular Weight: 25 kDa (Predicted band size）

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution., ICC staining DDIT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: REDD-1, DDIT4, REDD1, RTP801, DDIT4, DDIT4, Dig2, DNA damage inducible transcript 4, DNA damage inducible transcript 4 protein, DNA damage-inducible transcript 4 protein, FLJ20500, HIF 1 responsive protein RTP801, HIF 1 responsive RTP801, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1, REDD-1, REDD1, RTP801,

Immunogen: Peptide.

Conjugate: Unconjugated

Modification: Unmodified