Histone H4 (MonoMethyl-K12) Polyclonal Antibody

Catalogue Numbers: BS67159-50, BS67159-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P62805

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 120% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Histone H4 with a site at MonoMethyl-K12 protein.

Extra Notes: Western blot analysis of Histone H4 (MonoMethyl-K12) expression in HEK293T (A), H446 (B), H1688 (C) whole cell lysates.

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 with a site at MonoMethyl-K12. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified