GOLPH3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP6024-50, AP6024-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H4A6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. GOLPH3L (golgi phosphoprotein 3-like), also known as GPP34R, is a 285 amino acid cytoplasmic protein that localizes to the Golgi apparatus. Belonging to the GOLPH3/VPS74 family, GOLPH3L may have a regulatory role in Golgi trafficking. GOLPH3L is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GOLPH3 polyclonal antibody detects endogenous levels of GOLPH3 protein.

Bioworld Molecular Weight: ~ 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of GOLPH3 pAb at 1:2000 dilution
Lane1:SGC7901 whole cell lysate(40ug)
Lane2:3T3-L1 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:PC3 whole cell lysate(40ug)
Lane5:H9C2 whole cell lysate(40ug)

Alternative Name: Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS

Immunogen: Recombinant full length Human GOLPH3.

Conjugate: Unconjugated

Modification: Unmodified