FTβ (E285) Polyclonal Antibody

Catalogue Numbers: BS3377-50, BS3377-100

Sizes: 50µl, 100µl

Alternative Name: Protein farnesyltransferase subunit beta; FTase-beta; FTase-β; FTase β; CAAX farnesyltransferase subunit beta; Ras proteins prenyltransferase subunit beta; FNTB

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49356

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical α and β subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an a-b heterodimer. The β subunit, which is known as FTβ, CAAX farnesyltransferase subunit β, or Ras proteins prenyltransferase subunit β, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate. The α subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FTβ (E285) polyclonal antibody detects endogenous levels of FTβ protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FTβ (E285) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate(40ug)
Lane 2: PC3 whole cell lysate(40ug)
Lane 3: MCF-7 whole cell lysate(40ug)
Lane 4: SGC7901 whole cell lysate(40ug)
Lane 5: L02 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human FTβ.

Conjugate: Unconjugated

Modification: Unmodified