CHST1 Polyclonal Antibody

Catalogue Numbers: BS5662-50, BS5662-100

Sizes: 50µl, 100µl

Alternative Name: Carbohydrate sulfotransferase 1; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; KSST

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43916

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Carbohydrate sulfotransferase 1 (CHST1), also referred to as KSGal6ST or KSST, is a keratan sulfate sulfotransferase. It is predominantly expressed in brain and skeletal muscle and localizes to the trans-Golgi network. CHST1 is responsible for mediating the sulfation of keratan in the cornea, which is important in maintaining corneal transparency. In particular, CHST1 catalyzes the transfer of sulfate groups from 3’-phosphoadenosine 5’-phosphosulfate to position six of internal or terminal galactose residues (preferentially the residues adjacent to sulfated GlcNAc) on growing keratan sulfate chains. CHST1 also contributes to the generation of L-selectin ligands. Mutations in the CHST1 gene may play a role in macular corneal dystrophy (MCD).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CHST1 polyclonal antibody detects endogenous levels of CHST1 protein.

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CHST1 polyclonal antibody at 1:500 dilution
Lane 1: A549 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 126-171 of Human CHST1.

Conjugate: Unconjugated

Modification: Unmodified