Drosha Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90429-50, BS90429-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NRR4(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Drosha polyclonal antibody detects endogenous levels of Drosha protein.

Bioworld Molecular Weight: 159 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution., ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: RNase III, DROSHA, RN3, RNASE3L, RNASEN, DROSHA, Drosha double stranded RNA specific endoribonuclease, Drosha ribonuclease type III, Etohi2, HSA242976, Nuclear RNase III Drosha, p241, Protein Drosha, Putative protein p241 which interacts with transcription factor Sp1, Putative ribonuclease III, RANSE3L, Ribonuclease 3, Ribonuclease III, Ribonuclease III nuclear, Ribonuclease type III nuclear, RibonucleaseIII, RN 3, RN3, RNase 3, RNase III, RNase3, RNASE3L, RNaseIII, RNASEN, RNC,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified