Recombinant Human SerpinA3/AACT Protein (His Tag)(Active)

Size: 100μg

Catalogue Number: PKSH031694-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: SerpinA3; AACT

Target Symptom: Alpha-1-Antichymotrypsin; ACT; Cell Growth-Inhibiting Gene 24/25 Protein; Serpin A3; SERPINA3; AACT; GIG24; GIG25; MGC88254; SERPINA3

Research Area: Cell biology; Cardiovascular; Neuroscience

Target Species: Human

Expression Host: HEK293 Cells

Application: Enzyme

Fusion Tag: C-His

Accession: NP_001076.2

Background: SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. In addition, ACT gene polymorphism has been implicated with Alzheimer’s disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.

Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog# 27114). The IC50 value is < 5 nM.

Sequence: Met 1-Ala 423

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 25mM HEPES, 0.15M NaCl, pH 7.8
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 46.5 kDa

Observed MW: 45 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only