NPM1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0086-50, MB0086-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06748

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, ICC

All Applications: WB: 1:1000
ICC: 1:100~200

Background: Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of NPM1 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 33KDa
Observed band size: 38KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of NPM1 antibody in Hela, Jurkat and 3T3 cell lysates using NPM1 antibody (1:1000 diluted)., Immunocytochemistry of HeLa cells using anti- NPM1 antibody diluted 1:200

Alternative Name: Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM

Immunogen: Recombinant full length Human NPM1.

Conjugate: Unconjugated

Modification: Unmodified