CD27 Polyclonal Antibody

Catalogue Numbers: NCP0197P-50, NCP0197P-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q5ZPR3

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:2000~1:5000

Background: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD27 polyclonal antibody detects endogenous levels of CD27 protein.

Extra Notes: Western blot (WB) analysis of CD27 polyclonal antibody at 1:2000 dilution
Lane1:A549 cell membrane lysate(25ug)
Lane2:A375 cell membrane lysate(25ug)

Bioworld Molecular Weight: ~90 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD276

Immunogen: Recombinant protein of human CD27.

Conjugate: Unconjugated

Modification: Unmodified