Recombinant Human ACLY/acly/ATP citrate lyase Protein (His Tag)

Size: 50μg

Catalogue Numbers: PKSH031004-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: ACLY; acly; ATP citrate lyase

Target Synonym: ACL; ATPCL; CLATP

UNIProt ID: P53396

Research Areas: Signal Transduction; Cancer; metabolism;

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: N-His

Accession: P53396

Background: ATP citrate lyase, also known as Acly or Acl, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is composed of two polymer chains which are polypeptides in human. ATP citrate lyase is responsible for catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In vivo, it reduces tumor growth leading to a cytostatic effect and induces differentiation.

Sequence: Met 1-Met 1101

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 123 kDa

Observed MW: 110 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only