AFP Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9042-50, MB9042-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02771(Human)

Host: Mouse

Reactivity: Human

Applications: WB, IHC ICC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: AFP monoclonal antibody detects endogenous levels of AFP protein.

Bioworld Molecular Weight: 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution., Western blot analysis of AFP on HepG2 cell lysate using anti-AFP antibody at 1/1,000 dilution.

Alternative Name: HPAFP, AFP, Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA, Hereditary persistence of alpha fetoprotein, HPAFP,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified