Topo IIα Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA30602-50, RA30602-100

Citations, Manuals and MSDS Available upon request.

Background: topoisomerase (DNA) II alpha (TOP2A) Homo sapiens This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Alternative Names: TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme

Applications: WB; IHC-p; IF/ICC; ELISA

Species Cross-Reactivity: Human; Monkey

GeneID (Human): 7153

Protein MW (KDa): 174

SWISS: P11388

Source: Rabbit

Research Use Only