Histone H2A.Z Polyclonal Antibody

Catalogue Numbers: BS67533-50, BS67533-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P0C0S5

Host: Rabbit

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Sheep

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Modulation of chromatin structure plays a critical role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure . There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z . Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability . Several homologous protein complexes, such as SWR-C (S. cerevisiae), TIP60 (D. melanogaster) and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This exchange of histone H2A variants changes histone-histone interactions in the nucleosome core and alters an acidic patch on the surface of the nucleosome, resulting in changes in nucleosome stability and binding of non-histone proteins such as HP1α .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Histone H2A.Z protein.

Extra Notes: Western blot analysis of Histone H2A.Z expression in DLD (A) whole cell lysates.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: H2AZ; Histone H2A.Z; H2A/z

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2A.Z. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified