Grp75 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9255-50, MB9255-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P38646(Human) P38647(Mouse) P48721(Rat)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Grp75 monoclonal antibody detects endogenous levels of Grp75 protein.

Bioworld Molecular Weight: 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: HepG2
Lane 3: K562
Lane 4: L929, ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GRP-75, MOT, PBP74, HSPA9, GRP75, HSPA9B, mt-HSP70, 75 kDa glucose regulated protein, 75 kDa glucose-regulated protein, CSA, Glucose Regulated Protein, Grp 75, GRP-75, GRP75, GRP75, Heat shock 70 kDa protein 9, Heat shock 70kD protein 9, heat shock 70kDa protein 9, Heat shock 70kDa protein 9B, Heat shock protein 74 kDa A, Heat shock protein A, Heat shock protein cognate 74, Hsc74, Hsp74, Hsp74a, HSPA9, Hspa9a, HSPA9B, MGC4500, mitochondrial, Mortalin 2, Mortalin, Mortalin perinuclear, Mortalin2, MOT 2, MOT, MOT2, Mthsp70, p66 mortalin, P66 MOT, PBP74, Peptide binding protein 74, Peptide-binding protein 74, Stress 70 protein mitochondrial, Stress 70 protein mitochondrial precursor, Stress-70 protein,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified