MYOD1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66892-50, MB66892-100

Product: Mouse IgM. Supplied in crude ascites with 0.01% sodium azide.

Swiss-Prot: P15172

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Myoblast determination protein 1, also called myogenic factor 3 , is a member of the MyoD family of muscle specific bHLH transcription factors . This family is responsible for controlling specification of the muscle cell lineage and members are expressed only in skeletal muscle and its precursors. MyoD1 is considered a master regulator of skeletal myogenesis as its expression can induce myogenic differentiation in myoblasts, fibroblasts, and a variety of other cell types . Through ChIP-sequencing experiments, researchers have discovered that MyoD is associated with the promoters of many genes in muscle cells, but it only regulates a subset of those genes. These research studies point to regulation of MyoD transcriptional activity via epigenetic mechanisms involving SWI/SNF complexes and Polycomb and Trithorax Group proteins . Additional influences on muscle development include signal transduction through MAPK, PI3K/Akt, myostatin, NF-κB, and mTOR signaling pathways.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MYOD1 protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MYOD1 expression in A549 (A) whole cell lysates., ???, ???, ???

BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MYOD1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified