S100-B Monoclonal Antibody

Catalogue Numbers: MB66795-50, MB66795-100

Sizes: 50µl, 100µl

Product: Mouse IgM kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P50114

Host: Mouse

Reactivity: Mouse

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 a and b, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin a and parvalbumin b (also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 a chain and S-100 b chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two a chains, two b chains or one a and one b chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann’s cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 a and b are present in a variety of other tissues, and calbindin is present in intestine and kidney.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of S100-B protein.

Extra Notes: Western blot analysis of S100-B expression in mouse liver (A) whole cell lysates.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

Immunogen: Recombinant fusion protein of human S100-B. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified